QuickAntibody-Mouse5W（5周标准鼠单抗/多抗制备佐剂）

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QuickAntibody-Mouse5W（5周标准鼠单抗/多抗制备佐剂）

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QuickAntibody-Mouse5w

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Catalog_no	KX0210041
Product_name	QuickAntibody-Mouse5W（5周标准鼠单抗/多抗制备佐剂）
Size	1ml
Category	免疫佐剂
Price	640.00
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QuickAntibody-Mouse5W （5周标准鼠单抗/多抗制备佐剂）

QuickAntibody-Mouse5W

用途

QuickAntibody-Mouse5W

使用方法（详见使用说明书）

如何选择

QuickAntibody

系列免疫佐剂？

产品名称	货号	规格	免疫周期	特点	用途	预期滴度
QuickAntibody -Mouse5W	KX0210041	1ml	5周	标准免疫	小鼠单抗小鼠多抗	1:10000- 1:10000000
QuickAntibody -Mouse3W	KX0210042	1ml	3周	快速免疫	小鼠单抗小鼠多抗	1:10000- 1:100000
QuickAntibody -Mouse2W	KX0210043	1ml	2周	快速免疫	小鼠多抗	1:1000- 1:10000
QuickAntibody -Rabbit8W	KX0210045	1ml	8周	标准免疫	兔多抗	1:10000- 1:1000000

QuickAntibody

系列免疫佐剂有哪些特点？

1. 丁宁, 石心泉, 郭永, 张瑶楠, 邹检平. 一种快速制备甲胎蛋白单克隆抗体杂交瘤细胞系的方法, 生殖医学杂志, 20l3年5月第22卷第5期.

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2. 廖四芳, 刘丹, 宋文, 刘明, 赵阳, 唐超, 吴秀山, 王跃群. 抗果蝇MRJ 蛋白单克隆抗体的制备和鉴定, 激光生物学报, 2013, 22(2).

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3. Xindi Ruan, Shaomei Huang, Lin Shao, Jing Ye, Zheng Chen, Huanchun Chen, and Shengbo Cao. Monoclonal antibodies against NS4B protein of Japanese Encephalitis Virus,Monoclonal Antibodies in Immunodiagnosis and Immunotherapy,2013, 32(6):382-5.

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4. 余涛, 李大刚, 邓宁, 向军俭, 宋其芳, 贾彦琼, 王宏. 抗玉米赤霉烯酮单抗独特型抗体制备与鉴定, 食品科学, 2013, 03(34):184.

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5. Xiujin Chen, Liqiang Liu, Hua Kuang, Shanshan Song and Chuanlai Xu. A strip-based immunoassay for rapid determination of fenpropathrin, Anal. Methods, 2013, 5, 6234.

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6. Xun Zhang, Min Feng, Liqiang Liu, Changrui Xing, Hua Kuang, Chianfang Peng, Libing Wang, Chuanlai Xu. Detection of aflatoxins in tea samples based on a class-specific monoclonal antibody, International Journal of Food Science and Technology 2013,1-6. (小分子抗原)

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7. Xun Zhang, Liqiang Liu, Xiujin Chen, Hua Kuang, Shanshan Song, Chuanlai Xu. Immunochromatographic Strip Development for Ultrasensitive Analysis of Aflatoxin M1, Anal. Methods, 2013, 5, 6567.

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8. Xiao Zhang, Yuzhen Zhang, Junjun Liu, Honglei Liu. PotD protein stimulates biofilm formation by Escherichia coli, Biotechnol Lett 2013, 35:1099–1106.

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9. Yongji He, Wenli Ma, Yingjun Li, Jinping Liu, Weixin Jing, Lan Wang Y. He, W. Ma, Y. Li, J. Liu, W. Jing, L. Wang. Expression of metallothionein of freshwater crab (Sinopotamon henanense) in Escherichia coli enhances tolerance and accumulation of zinc, copper and cadmium Ecotoxicology, 2013, 27.

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10. Miao Zhao, Yu Bai, Wei Liu, Xiangqian Xiao, Yuming Huang, Shan Cen, Paul KS Chan, Xin Sun,Wang Sheng, Yi Zeng. Immunization of N terminus of enterovirus 71 VP4 elicits cross-protective antibody responses. BMC Microbiology 2013, 13:287.

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11. Zhuo Zhao, Bin Li, He-Qiang Sun, Jin-Yong Zhang, Yi-Lin Wang, Li Chen, Jian Hu, Ya-Fei He, Hao Zeng, Quan-Ming Zou, Chao Wu. Fine-mapping of immunodominant linear B-cell epitopes of the Staphylococcus Aureus SEB antigen using short overlapping peptides, PLOS ONE, March 2014,Volume 9, Issue 3, e90445.

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12. 李松, 杨立彬, 张舒岩, 金羽, 陈秀华, 李新, 刘玲, 李树蕾, 李树红. 建鲤重组Cystatin蛋白的原核表达、鉴定及多克隆抗体制备. 吉林大学学报 (医学版), 2014年01期. (小鼠多抗)

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13. 李琳. P. fluorescens脂肪酶(Lip)酶活特性研究及分泌抗Lip杂交瘤细胞株的制备. 哈尔滨工业大学硕士学位论文, 2013, 6.

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14. 孙凤霞. 三聚氰胺单克隆抗体及其高灵敏快速检测技术研究. 江南大学博士论文, 2011, 12. (小分子抗原)

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15. 郭纪珂, 戈曼, 张泽力, 马建, 申之义, 王晓钧. 抗马传染性贫血病毒Rev蛋白单克隆抗体的制备. 中国预防兽医学报, 2014, 36(2): 157-9.

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16. 郭纪珂. 马传染性贫血病毒Rev蛋白单克隆抗体的制备及表位鉴定. 内蒙古农业大学硕士学位论文, 2014, 6.

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17. 贾鹏, 钟松清, 何俊强, 刘荭, 郑晓聪, 谭攀, 王津津, 史秀杰, 兰文升, 于力, 谢冬霞. 抗IHNV单克隆抗体6G7及其制备与应用, 中国发明专利申请号CN201310433433, 公开号CN103525767A.

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18. 郑晓聪, 钟松清, 何俊强, 谭攀, 贾鹏, 王津津, 史秀杰, 兰文升, 于力, 谢冬霞, 刘荭. 抗SVCV单克隆抗体3E1及其制备与应用, 中国发明专利申请号CN103509758A, 公开号201310433480.6.

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19. 王旻, 涂孝洁, 何花, 张娟, 罗晨. 抗CD24单克隆抗体、其可变区序列及其应用, 中国发明专利申请号CN201410027795, 公开号CN103819561A.

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20. 郑晓聪, 钟松清, 何俊强, 谭攀, 贾鹏, 王津津, 史秀杰, 兰文升, 于力, 刘荭, 谢冬霞. 抗SVCV单克隆抗体4F9及其制备与应用, 中国发明专利申请号CN201310433446, 公开号CN103525768A.

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21. 贾鹏, 钟松清, 何俊强, 郑晓聪, 谭攀, 王津津, 刘荭, 史秀杰, 兰文升, 于力, 谢冬霞. 抗IHNV单克隆抗体5H3及其制备与应用, 中国发明专利申请号CN201310433521, 公开号CN103509759A.

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22. 匡华, 胥传来, 张勋, 徐丽广, 马伟, 刘丽强, 宋珊珊, 吴晓玲. 一株抗黄曲霉毒素通用型单克隆抗体杂交瘤细胞株及其应用, 中国发明专利申请号CN201410214953, 公开号 CN104004717A. (小分子抗原)

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23. 刘召民, 山东大学硕士论文. 青岛文昌鱼ERK5, p38抗体的制备及体内蛋白表达的初步研究, 2012.5.

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24. 何永吉, 刘进平, 李颖君, 王兰华溪蟹功能性重组金属硫蛋白的表达及抗血清制备, 环境科学学报, 2014, 34(9): 2434-2439.

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25. 刘永飞, 天津大学研究生论文. A型流感病毒蛋白与宿主蛋白相互作用研究, 2012.12.

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26. 郭颖, 中科院海洋所研究生论文. 栉孔扇贝脑啡肽及其受体介导的神经内分泌免疫调节, 2013.5.

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27. 王旻, 王泽根, 涂孝洁, 金海珍, 厉道娟, 许卓斌, 张娟, 吴旻. 一种抗人Delta like4单克隆抗体, 中国发明专利申请号CN201410079857, 公开号 CN103804497A.

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28. 翟晋豫. 河南师范大学硕士论文, 胰腺癌等肿瘤标志物(CA19-9)单克隆抗体制备及检测方法的初步建立, 2014.5.

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29. 王云龙. 糖类抗原19-9单抗制备及DAS-ELISA的建立, 中国免疫学杂志, 2014, 30(8): 1088.

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30. 何永吉. 河南华溪蟹金属硫蛋白单克隆抗体的制备与鉴定, 细胞与分子免疫学杂志, 30(8): 844.

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31. 王晓雯. 北京工业大学博士论文, 高致病小RNA病毒病毒样颗粒的制备及部分颗粒的免疫研究, 2013.6.

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32. 陈秀金. 江南大学博士论文, 拟除虫菊酯类农药免疫快速检测方法及其定量构效模型的建立, 2013.6. (小分子抗原)

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33. 张勋. 江南大学博士论文, 真菌毒素类高灵敏高通量快速检测方法研究, 2014.6. (小分子抗原)

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34. 赵淼. 北京工业大学博士论文, 手足口病预防性基因工程重组疫苗的研究, 2014.6.

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35. 张慧茹, 翟晋豫, 刘珍, 王雪琴, 孟素香. 角蛋白19片段单克隆抗体的制备及鉴定, 动物医学进展, 2014, 35(12): 5-9.

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36. 杨健, 刘冬梅, 何永吉, 汉斯达姆斯, 王兰. 镉对华西蟹卵黄磷蛋白在卵巢中表达含量的影响及ELISA法的建立, 水生生物学报, 2015, 39(2): 287-293.

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37. Qian Dong, Qiuping Xu, Xindi Ruan, Shaomei Huang, Shengbo Cao, Monoclonal Antibodies Against NS2B of Japanese Encephalitis Virus. Monoclonal Antibodies Immunodiagnosis and Immunotherapy, 2015, 34 (2): 96-100.

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38. Zeming Li, Qi Zhang, Peixiao Luo, Guangxin Liu, Min Wang, Xueqin Liu, Monoclonal Antibodies Against M Protein of Spring Viremia of Carp Virus. Monoclonal Antibodies Immunodiagnosis and Immunotherapy, 2015, 34 (2): 122-125.

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39. 兰美静. 浙江大学硕士论文, 农药多残留免疫芯片-纳米金增敏型检测体系构建, 2015.6.

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40. 徐乃丰. 江南大学博士论文, 水产品中三类重要化学残留物快速检测方法的研究, 2015.6.

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41. 李树红, 陈志光, 李冉, 李新, 李松, 陈秀华, 蒋然然, 李美良, 马璐阳. 建鲤组织蛋白酶L的原核表达、纯化鉴定及多克隆抗体的制备, 中国水产科学, 2015, 22(5): 849-857. (小鼠多抗)

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42. Xingui Tian, Qiang Ma, Zaixue Jiang, Junfeng Huang, Qian Liu, Xiaomei Lu, Qingming Luo, and Rong Zhou, Identification and Application of Neutralizing Epitopes of Human Adenovirus Type 55 Hexon Protein. Viruses, 2015, 7: 5632-5642.

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43. 薛添. 西北大学硕士论文, 汉滩病毒重组假病毒的构建及包膜蛋白糖基化对病毒免疫学性状影响的初步研究, 2015.4.

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44. 王士峰, 张世伟, 赖心田, 冯荣虎, 伍聪, 杨国武. 高灵敏间接竞争ELISA检测羊乳样本中的牛乳成分, 中国乳品工业, 43(10): 2015, 41-43.

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45. Mengqiang Wang, Lingling Wang, Ying Guo, Rui Sun, Feng Yue, Qilin Yi, Linsheng Song. The broad pattern recognition spectrum of the Toll-like receptor in mollusk Zhikong scallop Chlamys farreri, Developmental and Comparative Immunology 2015 (52): 192-201. (免疫大鼠)

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46. Qiang Ma, Xingui Tian, Zaixue Jiang, Junfeng Huang, Qian Liu, Xiaomei Lu, Qingming Luo, Rong Zhou. Neutralizing epitopes mapping of human adenovirus type 14 hexon, Vaccine 2015 (33): 6659-6665.

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47. Hua He, Xiaojie Tu, Juan Zhang, Desmond Omane Acheampong, Li Ding, Zhaoxiong Ma, Xueyan Ren, Chen Luo, Zhiguo Chen, Tong Wang, Wei Xie, Min Wang. A novel antibody targeting CD24 and hepatocellular carcinoma in vivo by near-infrared fluorescence imaging, Immnuobiology 2015 (220): 1328-1336.

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48. 郭丽茹, 李晓燕, 孔梅, 刘英华, 邹明, 苏旭. 人偏肺病毒F1、F2亚单位原核表达系统的构建及多克隆抗体制备, 疾病监测, 2015 30 (11): 964-968. (QuickAntibody-Mouse2W)

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49. 杨宇琴, 王细花, 吕悦, 程广宇, 周向梅. beta防御素在牛健康组织和病变组织中的分布, 畜牧与兽医, 2015, 47 (12): 25-30. (QuickAntibody-Rabbit8W)

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50. 孔德生. 哈尔滨兽医研究所硕士论文, 不同亚型兔出血症病毒单克隆抗体的制备及抗原表位鉴定, 2015.5.

51. 张莉莉. 安徽医科大学硕士论文, 玻连蛋白单克隆抗体的制备及在临床检测中的初步应用, 2015.5.

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52. 董倩. 华中农业大学硕士论文, JEV NS2B蛋白单克隆抗体的制备及其应用, 2015.6.

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53. 刘倩, 史利宁, 商秀娟, 陈笑, 胡毓安, 陈勇, 李芳秋. 烟曲霉硫氧还蛋白还原酶对侵袭性曲霉病小鼠的免疫保护作用, 中国真菌学杂志, 2015, 10 (6): 340-344.

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54. Desheng Kong, Jiasen Liu, Qian Jiang, Zuo Yu, Xiaoliang Hu, Dongchun Guo, Qianqian Huang, Meihui Jiao, Liandong Qu, Production, Characterization, and Epitope Mapping of Monoclonal Antibodies Against Different Subtypes of Rabbit Hemorrhagic Disease Virus (RHDV), Scientific Reports 2016 DOI: 10.1038/srep20857.

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55. Xiaowen Wang, XiangQian Xiao, Miao Zhao, Wei Liu, Lin Pang, Xin Sun, Shan Cen, Burton B. Yang, Yuming Huang, Wang Sheng and Yi Zeng. EV71 virus-like particles produced by co-expression of capsid proteins in yeast cells elicit humaoral protective response against EV71 lethal challenge, BMC Res Notes 2016 9:42.

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56. Zhiwei Wang, Yunhang Guo. Anti-PD1 Antibodies and Their Use as Therapeutics and Diagnostics, WO 2015/035606.

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57. Zhuobin Xu, Zegen Wang, Xuelian Jia, Luxuan Wang, Zhiguo Chen, Shijing Wang, Min Wang, Juan Zhang, Min Wu. MMGZ01, an anti-DLL4 monoclonal antibody, promoters nonfunctional vessels and inhibits breast tumor growth, Cancer Letter 2016 372:118-127.

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58. Rui Liu, Ying Liu, Meijing Lan, Niusha Taheri, Jingli Cheng, Yirong Guo, Guonian Zhu. Evaluation of a water-soluble adjuvant for the development of monoclonal antibodies against small-molecule compounds, , Journal of Zhejiang University-Sci B (Biomed & Biotechnol) 2016 ISSN 1673-1581 (Print); ISSN 1862-1783 (Online).

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59. 张晓光, 杨韧, 王娇, 王轩, 侯美玲, 安丽娜, 朱莹, 曹玉玺, 曾毅. 埃博拉病毒重组膜蛋白Gp-Fc表达及免疫原研究. 病毒学报,2016,, 32(1):8-13.

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60. 欧小蕾. 广西海洋大学硕士论文, 膝沟藻毒素GTX2,3单克隆抗体的制备及酶联免疫快速检测法的建立, 2015.6.

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61. 李晓燕,孔梅,郭丽茹,杨东靖,苏旭,刘英华,邹明,吕莉琨. 人偏肺病毒多表位抗原的构建与表达. 环境与健康杂志,2015,32(11): 977-983.

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62. 杨健. 山西大学博士论文. 镉对河南华溪蟹脂肪积累与代谢的影响及毒性机制研究. 2015.6.

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63. 张梅, 邱郑, 邢黎军, 强旭, 张娟, 王昊. 叶酸受体a原核载体的构建及表达，药物生物技术，2016,23(1): 26-29.

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64. Yujun Zhou, Yuanye Zhu, Yanjun Li, Yaing Duan, Rongsheng Zhang, Mingguo. beta1 tubulin rather than beta2 tubulin is the preferred binding target for carbenbazim in Fusarium, Phytopathology, 2016 May 2 ([Epub ahead of print] Online).

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65. 陈尧, 王兴华, 何永吉, 潘薇薇, 曹鹏程, 赵峰梅, 张全爱, 赵邑. 具有抗凝活性抗人组织因子单克隆抗体的制备与鉴定. 细胞与分子免疫学杂志, 2016, 32(4): 544-548.

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66. 赖心田, 冯荣虎, 张世伟, 王士峰, 杨国武. 间接竞争ELISA检测核桃制品中核桃蛋白含量, 食品工业, 2016,37(4): 291-294.

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67. 赵小磊, 翟晋豫, 刘玲玲, 梁永波, 牛银银, 李三华, 齐华. 孕激素受体A型原核表达及单克隆抗体制备, 中国免疫学杂志, 2016, 32: 1013-1016.

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68. 王丹. 中国农业大学博士论文. 鸭源呼肠孤病毒的表型和分子特征分析及基因2型毒株编码的新蛋白的鉴定. 2016.5. （免疫兔）

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69. Mengqiang Wang, Lingling Wang, Mengmeng Huang， Qilin Yi，Ying Guo, Yunchao Gai, Hao Wang, Huan Zhang, Linsheng Song. A galectin from Eriocheir sinensis functions as pattern recognition receptor enhancing microbe agglutination and haemocytes. Fish & Shellfish Immunology 2016,55: 10-20. （免疫大鼠）

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70. 宋军营，张钟允，刘文弟，曾华辉，孙向东，闫敏，袁永，翟晋豫. 人抗P53蛋白单克隆抗体的制备及评价. 免疫学杂志, 2016, 32(8): 708-711.

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71. 杨金亮, 姚于勤, 陈小新, 龙超峰, 抗血小板衍生因子的抗体, 中国发明专利申请号CN105732809A, 公开号 CN103804497A.（蛋白抗原单抗制备）

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72. Shaozheng Song, Zhengyi He, Junyan Mei, Zhengqiang Qi,Si Chen, Sheng Xu, Yuguo Yuan, Yong Cheng. Affinity purification of recombinant human plasminogen activator from transgenic rabbit milk using a novel polyol-responsive monoclonal antibody. Tropical Journal of Pharmaceutical Research 2016,15(5):905-911. （蛋白抗原单抗制备）

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73. 杨样. 扬州大学硕士论文, 产肠毒素大肠杆菌溶血素HlyA的原核表达、多抗制备及相关功能探析, 2015.6. （蛋白抗原多抗制备）

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74. 宋绍征. 扬州大学博士论文, 转基因兔乳腺特异性表达重组人纤溶酶原激活剂（rhPA）及其药效学研究, 2016.6 （单抗制备,与弗氏佐剂做了对比）

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75. 赵志腾. 东北农业大学硕士论文, 山东省貂源铜绿假单胞菌的分离鉴定及三价灭活菌对小鼠的免疫效力评价 2016.6 （兔多抗制备,全菌抗原）

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76. 尹颖. 扬州大学硕士论文, 针对禽流感N5, N8, N9三种神经氨酸酶NA蛋白单克隆抗体及N5胶体金试纸条的研制 2016.6 （单抗制备,病毒抗原）

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77. 吴晓鑫. 浙江大学硕士论文, 埃博拉病毒GP蛋白单克隆抗体的筛选和制备 2015.12 （单抗制备,病毒抗原）

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78. 李星月. 东北农业大学硕士论文, 检测大肠杆菌菌毛的新型凝集技术研究 2016.6 （兔多抗制备, 菌毛抗原）

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79. 卿莉, 四川农大硕士论文, 基于密码子优化的VP1基因表达蛋白检测1型和3型鸭甲肝病毒抗体的胶体金试纸条, 2016.6（QuickAntibody-Mouse3W 小鼠多抗）

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80. 黄霞四川农大硕士论文鸭疫里默氏菌dnaK基因功能初析, 2016.6（QuickAntibody-Mouse3W 小鼠多抗）

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81. 张岱浙江大学硕士论文鸟样中柠檬黄与乙烯雌酚ELISA检测方法的建立和随机人群暴露水平分析, 2017.1（单抗制备小分子抗原乙烯雌酚）

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82. 曹靖雨四川农大硕士论文 1型鸭甲肝病毒2A蛋白功能初探, 2016.6（QuickAntibody-Mouse2W 小鼠多抗制备多肽抗原）

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83. 陈曦, 牛支原体HB0801株VspX蛋白单克隆抗体的制备与鉴定, 中国畜牧兽医, 2017, 44(3): 868-878.

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84. 孙丹丹华侨大学硕士论文 Kallistatin蛋白的哺乳动物表达、纯化及单克隆抗体制作初探, 2016.3.

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85. 胥传来一株抗吡利霉素通用单克隆抗体杂交瘤细胞株及其应用发明专利授权 CN201410260782.2

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86. Chao Zhang, Yaping Gu, Jianxia Tang, Feng Lu, Yuanyuan Cao, Huayun Zhou, Guoding Zhu, Jun Cao, Qi Gao, Production of Plasmodium vivax Enolase in Escherichia coli and its Protective Properties, Human Vaccines & Immunotherapeutics, DOI: 10.1080/21645515.2016.1208328.（QuickAntibody 小鼠多抗中和实验）

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87. Niusha Taheri, Meijing Lan, Peng Wei, Rui Liu, Wenjun Gui, Yirong Guo, Guoniian Zhu, Chemiluminescent Enzyme Immunoassay for Rapid Detction of Three alpha-Cyano Pyrethroid Residues in Agricultural Products, Food Anal. Methods, DOI 10.1007/s12161-016-0482-x, 2016.（QuickAntibody-Mouse5W 单抗小分子抗原）

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88. Jian Yang, Hui Sun, Hao Zhang, Hui Zhou, Expression, purification of metallothionein genes from freshwater crab (Sinopotamon yangtsekiense) and development of an anti-metallothionein ELISA, PLOS ONE, March, 2017.（QuickAntibody 小鼠多抗）

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89. Shu-Hong Li, Ran Li, Hai-Xia Zhong, Xiu-Hua Chen, Ai-Ping Liu, Juan Yang, Qiang Hu, Qin Ke, and Mei-Liang Li, Immunolocalization of Jian Carp (Cyprinus Carpio Var. Jian) Cathepsin B: Cloning, Expression, Characterization, and Antibody Preparation, Journal of Food Science, 2017, DOI: 10.1111/1750.3841.13708.（QuickAntibody-Mouse5W 小鼠单抗 WB IHC）

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90. Leijun Yao, Liqiang Liu, Shanshan Song, Hua Kuang, Chuanlai Xu, Development of indirect competitive enzyme-liked immunosorbent and immunochromatographic strip assays for carbofuran detection in fruits and vegetables, Food and Agricultural Immunology, 2017, DOI: 10.1080/09540105.2017.1309359.（QuickAntibody-Mouse5W 单抗制备小分子抗原）

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91. 余厚美,安飞飞,罗秀芹,陈松笔,欧文军. 淀粉磷酸化酶原核表达及单克隆抗体的制备. 中国农学通报, 2017,33(14):28-32.（QuickAntibody-Mouse5W 小鼠单抗制备）（含Clone Easy）

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92. 吴峰,吕琦,毛茅,岑瑜,马岚抗甲型流感病毒H1N1亚型单克隆抗体制备及应用. 云南大学学报（自然科学版）, 2017, 39(S1):106-110.（QuickAntibody-Mouse5W 小鼠单抗制备）（含Clone Easy）

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93. 张世伟，冯荣虎，王士峰，赖心田，杨国武，基于单克隆抗体的食品中乳酸链球菌素酶联免疫方法,中国食品添加剂,2017,8:166-169.（QuickAntibody-Mouse5W 小鼠单抗制备）

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94. ShanshanSong, Steven Suryoprabowo, Liqiang Liu, Hua Kuang, Liguang Xu,Wei Ma. Development of monoclonal antibody-based colloidal gold immunochromatographic assay for analysis of halofuginone in milk. Food and Agricultural Immunology Volume 30, 2019 - Issue 1

95. Liqiang Liu, Liguang Xu,Steven Suryoprabowo,Shanshan Song,Hua Kuang. Development of an immunochromatographic test strip for the detection of ochratoxin A in red wine. Food and Agricultural Immunology . Volume 29,2018-lssue 1.

96. Chengjie Xie, Rongzhi Wang, Abdullah F. U. H. Saeed, Qinghai Yang, Huiling Chen, Sumei Ling, Shiwei Xiao, Linmao Zeng & Shihua Wang. Preparation of Anti-Human Podoplanin Monoclonal Antibody and its application in Immunohistochemical Diagnosis. scientific reports
8, Article number: 10162 (2018).

97. Danping Zhang, Chengjie Xie, Rongzhi Wang, Qinghai Yang, Huiling Chen, Sumei Ling, Shihua Wang & Kunzhi Jia. Effective preparation of a monoclonal antibody against human chromogranin A for immunohistochemical diagnosis. BMC Biotechnology volume 18, Article number: 25 (2018)

98. Kai Hao,Steven Suryoprabowo,Shanshan Song,Liqiang Liu &Hua Kuang.Rapid detection of zearalenone and its metabolite in corn flour with the immunochromatographic test strip.
Food and Agricultural Immunology Volume 29, 2018 - Issue 1

99. Chunyan Yi, Xiaoyu Sun, Jing Ye, Longfei Ding, Meiqin Liu, Zhuo Yang, Xiao Lu, Yaguang Zhang, Liyang Ma, Wangpeng Gu, Aidong Qu, Jianqing Xu, Zhengli Shi, Zhiyang Ling & Bing Sun.
Key residues of the receptor binding motif in the spike protein of SARS-CoV-2 that interact with ACE2 and neutralizing antibodies. Cellular & Molecular Immunology volume 17, pages621–630(2020)

100. Fan Yang,Yixin Xiao,Rufeng Lu,Bin Chen,Fumin Liu,Liyan Wang. Generation of neutralizing and non-neutralizing monoclonal antibodies against H7N9 influenza virus. Emerging Microbes & Infections Volume 9, 2020 - Issue 1.

101. Tingting Chen, Kan Liu, Jiangyao Xu, Tianying Zhan, Maixian Liu, Li Li, Zhiwen Yang, Shuping Yuan, Wenyi Zou, Guimiao Lin, Dennis A. Carson, Christina C. N. Wu, and Xiaomei Wang. Synthetic and immunological studies on the OCT4 immunodominant motif antigen-based anti-cancer vaccine. Cancer Biol Med. 2020 Feb 15; 17(1): 132–141.

102. Tianqiong He, Mingshu Wang, Xuelian Cao, Anchun Cheng, Ying Wu, Qiao Yang, Mafeng Liu, Dekang Zhu, Renyong Jia, Shun Chen, Kunfeng Sun, Xinxin Zhao & Xiaoyue Chen . Molecular characterization of duck enteritis virus UL41 protein. Virology Journal volume 15, Article number: 12 (2018)

103. Lin Xu ,Jianmin Wu ,Qi Li ,Yamei Wei,Zhizhou Tan,Jianqiu Cai,Huancheng Guo,Ling’en Yang,Xiaohong Huang,Jing Chen,Fuqiang Zhang ,Biao He ,Changchun Tu . Seroprevalence, cross antigenicity and circulation sphere of bat-borne hantaviruses revealed by serological and antigenic analyses. PLoS Pathog 15(1): e1007545.Published: January 22, 2019

104. 李晓燕郭丽茹孔梅邹明苏旭. 人偏肺病毒多表位抗原的小鼠免疫应答. 中华微生物学和免疫学杂志２０１５年９月第３５卷第９期

105. 王英姿，闫剑勇，张世伟. 基于异源包被的曲安奈德竞争酶联免疫检测方法. 食品工业科技. Vol. 41,No.08,2020.

106. 于浩洋，吴绍强，王彩霞，Grzegorz WOZNIAKOWSKI，刘晓飞，仇松寅，李新实，冯春燕，林祥梅. 非洲猪瘟 CD2v 胞外区基因片段的真核表达及其多克隆抗体的制备. 中国兽医科学 . 2021，51（01)：45- 52

107. 吴峰，毛茅1，岑瑜，马岚. 抗 PⅠNP 单克隆抗体制备及应用. 云南大学学报 ( 自然科学版 ) ，2020，42( S2) : 101～ 104

108. 洪凌云，吴峰，毛茅，马岚. 抗 NTX 多克隆抗体制备及快速检测方法建立. 云南大学学报 ( 自然科学版 ) ，2020，42( S2) : 97～ 100

109. 王英姿，闫剑勇，张世伟. 基于异源包被的曲安奈德竞争酶联免疫检测方法. 食品工业科技. Vol.41,No.08,2020.

110. 王文青，蒋保余，曲自刚，刘保红，肖星星，马雪婷，许笑，蔡建平. 柔嫩艾美耳球虫穿孔素样蛋白基因的原核表达与亚细胞定位. 中国兽医科学 2019 ， 49(11) :1406-1414
111.Tingting Chen, Kan Liu, Jiangyao Xu, Tianying Zhan, Maixian Liu, Li Li, Zhiwen Yang, Shuping Yuan, Wenyi Zou, Guimiao Lin, Dennis A. Carson, Christina C. N. Wu, Xiaomei Wang,Synthetic and immunological studies on the OCT4 immunodominant motif antigen-based anti-cancer vaccine,Cancer Biology & Medicine,2020/2/15

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112.Yulin Xu, Mengxue Ye, Youwen Zhang, Shaohua Sun, Jia Luo, Sen Jiang, Jiajia Zhang, Xueliang Liu, Qi Shao, Qi Cao, Wanglong Zheng, François Meurens, Nanhua Chen, Jianzhong Zhu, Yulin Xu, Mengxue Ye,Screening of Porcine Innate Immune Adaptor Signaling Revealed Several Anti-PRRSV Signaling Pathways,Vaccines,2021/10/14

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113.Xingui Tian, Qiang Ma, Zaixue Jiang, Junfeng Huang, Qian Liu, Xiaomei Lu, Qingming Luo, Rong Zhou，Identification and Application of Neutralizing Epitopes of Human Adenovirus Type 55 Hexon Protein，Viruses-Basel，2015/10/27

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114.Yulin Xu, Mengxue Ye, Youwen Zhang, Shaohua Sun, Jia Luo, Sen Jiang, Jiajia Zhang, Xueliang Liu, Qi Shao, Qi Cao, Wanglong Zheng, François Meurens, Nanhua Chen, Jianzhong Zhu, Yulin Xu, Mengxue Ye, Youwen Zhang, Shaohua Sun, Jia Luo, Sen Jiang, Jiajia Zhang, Xueliang Liu, Qi Shao, Qi Cao, Wanglong Zheng, François Meurens,Nanhua Chen,Screening of Porcine Innate Immune Adaptor Signaling Revealed Several Anti-PRRSV Signaling Pathways，Vaccines，2021/10/14

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115.Li-Ping Gao, Yue-Zhang Wu, Kang Xiao, Xue-Hua Yang, Dong-Dong Chen, Qi Shi, Xiao-Ping Dong，Generation and characterization of two strains of transgene mice expressing chimeric MiniSOG-MusPrP，Journal Of Neuroscience Methods，2020/5/13

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116.Xiaoran Liu, Ya Li, Zhixin Li, Xiaomin Wei, Yanjie Ma, Peng Cheng, Rui Jiao, Jinzhi Fang, Yun Xing, Jiali Tang, Min Wang, Taiming Li，A novel IgG1 monoclonal antibody against xanthine oxidase alleviates inflammation induced by potassium oxonate in mice，International Journal Of Biological Macromolecules，2018/1/31

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117.Qiang Ma, Xingui Tian, Zaixue Jiang, Junfeng Huang, Qian Liu, Xiaomei Lu, Qingming Luo, Rong Zhou，Neutralizing epitopes mapping of human adenovirus type 14 hexon，Vaccine，2015/11/3

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118.Chunpeng Yang, Fang Zeng, Xinyu Gao, Shaojuan Zhao, Xuan Li, Sheng Liu, Na Li, Chenglin Deng, Bo Zhang, Rui Gong，Characterization of two engineered dimeric Zika virus envelope proteins as immunogen for neutralizing antibody selection and vaccine design，Journal Of Biological Chemistry，2019/5/28

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119.Liqiang Liu, Steven Suryoprabowo, Qiankun Zheng, Shanshan Song, Hua Kuang，Development of an immunochromatographic strip for detection of acetamiprid in cucumber and apple samples，Food And Agricultural Immunology，2017/4/5

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120.Kai Hao, Steven Suryoprabowo, Shanshan Song, Liqiang Liu, Hua Kuang,Rapid detection of zearalenone and its metabolite in corn flour with the immunochromatographic test strip,Food And Agricultural Immunology,2017/11/29

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121.Zhang, Danping, Xie, Chengjie, Wang, Rongzhi, Yang, Qinghai, Chen, Huiling, Ling, Sumei, Wang, Shihua, Jia, Kunzhi,Effective preparation of a monoclonal antibody against human chromogranin A for immunohistochemical diagnosis,Bmc Biotechnology,2018/5/4

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122.Lin Xu, Jianmin Wu, Qi Li, Yamei Wei, Zhizhou Tan, Jianqiu Cai, Huancheng Guo, Ling’en Yang, Xiaohong Huang, Jing Chen, Fuqiang Zhang, Biao He, Changchun Tu,Seroprevalence, cross antigenicity and circulation sphere of bat-borne hantaviruses revealed by serological and antigenic analyses,Plos Pathogens,2019/1/22

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123.Liu, Rui, Liu, Ying, Lan, Mei-jing, Taheri, Niusha, Cheng, Jing-li, Guo, Yi-rong, Zhu, Guo-nian,Evaluation of a water-soluble adjuvant for the development of monoclonal antibodies against small-molecule compounds,Journal of Zhejiang University-SCIENCE B,2016/4/14

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124. Zhengli Bai, Menglong Xu, Ying Mei, Tuo Hu, Panpan Zhang, Manman Chen, Wenxiu Lv, Chenchen Lu, Shuhua Tan, Zhengli Bai, Menglong Xu, Ying Mei, Tuo Hu, PanpanZhang, Manman Chen, Wenxiu Lv, Chenchen Lu,Generation of a Novel High-Affinity Antibody Binding to PCSK9 Catalytic Domain with Slow Dissociation Rate by CDR-Grafting, Alanine Scanning and Saturated Site-Directed Mutagenesis for Favorably Treating Hypercholesterolemia,Biomedicines,2021/11/27

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125.Shanshan Song, Steven Suryoprabowo, Liqiang Liu, Hua Kuang, Liguang Xu, Wei Ma, Xiaoling Wu,Development of monoclonal antibody-based colloidal gold immunochromatographic assay for analysis of halofuginone in milk,Food And Agricultural Immunology,2019/2/2

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126. Xie, Chengjie, Wang, Rongzhi, Saeed, Abdullah F. U. H., Yang, Qinghai, Chen, Huiling, Ling, Sumei, Xiao, Shiwei, Zeng, Linmao, Wang, Shihua,Preparation of Anti-Human Podoplanin Monoclonal Antibody and its application in Immunohistochemical Diagnosis,Scientific Reports,2018/7/5

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127.Jiao, Shasha, Liu, Pengyan, Liu, Ying, Zou, Rubing, Zhao, Ying, Liu, Yihua, Zhu, Guonian, Guo, Yirong,Binding properties of broad-specific monoclonal antibodies against three organophosphorus pesticides by a direct surface plasmon resonance immunosensor,Analytical And Bioanalytical Chemistry,2018/9/12

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128.Zhang, Jiaojiao, Li, Yujin, Wu, Xia, Zhong, Ruonan, Wei, Jing, Wang, Zhijian, Zhang, Xiaoping,Molecular structure, expression, and function analysis of BAFF gene in Chinese sucker, Myxocyprinus asiaticus,Fish Physiology And Biochemistry,2021/1/6

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129.Dai Zhang, Lifang Zhou, Yajing Lei, Zhan Zhou, Jie Zhou, Shuqing Chen,Investigation of diethylstilbestrol residue level in human urine samples by a specific monoclonal antibody,Environmental Science And Pollution Research,2017/1/14

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130.Yongji He, Lan Wang, Wenli Ma, Xiaoxia Lu, Yunlong Li, Jinping Liu,Secretory expression, immunoaffinity purification and metal-binding ability of recombinant metallothionein (ShMT) from freshwater crab Sinopotamon henanense,Ecotoxicology And Environmental Safety,2018/12/26

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131.Xiaola Guo, Xueyong Zhang, Jing Yang, Xiaoliang Jin, Juntao Ding, Haitao Xiang, Mazhar Ayaz, Xuenong Luo, Yadong Zheng,Suppression of nemo-like kinase by miR-71 in Echinococcus multilocularis,Experimental Parasitology,2017/10/13

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132.Ran Li, Shuhong Li, Zhiguang Chen, Yu Jin, Shulei Li, Song Li, Zhizi Bai,Grass carp (Ctenopharyngodon idella) stefin A: Systematic research on its cloning, expression, characterization and tissue distribution,Food Chemistry,2020/7/18

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133.Yi, Chunyan, Sun, Xiaoyu, Ye, Jing, Ding, Longfei, Liu, Meiqin, Yang, Zhuo, Lu, Xiao, Zhang, Yaguang, Ma, Liyang, Gu, Wangpeng, Qu, Aidong, Xu, Jianqing, Shi,Zhengli, Ling, Zhiyang, Sun, Bing,Key residues of the receptor binding motif in the spike protein of SARS-CoV-2 that interact with ACE2 and neutralizing antibodies,Cellular & Molecular Immunology,2020/5/15

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134.Yunhe Wang, Mengying Ding, Huaqing Ma, Jiao Wu, Hongwei Zhao, Yinglang Wan，Development of a specific monoclonal antibody-based icELISA for detection of arecoline in traditional Chinese medicines and fresh areca nuts，Food And Agricultural Immunology，2022/2/4

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物种	佐剂类型	抗原用量ug	抗原体积ul	佐剂体积ul
小鼠	QuickAntibody-Mouse	5~40ug	50	50
大鼠、豚鼠	QuickAntibody-Mouse	20~50ug	70	70
兔子、鸡	QuickAntibody-Rabbit	50~100ug	100	100
山羊、羊驼	QuickAntibody-Rabbit	200~500ug	300~500	300~500
猴子	QuickAntibody-Rabbit	200ug	200~300	200~300

溶剂	能否使用
PBS、生理盐水	可以
含有尿素	没有影响，客户反馈最高尿素可以高达6M以上
含有盐酸胍	没有影响
含有咪唑	没有影响，但是建议用PBS透析后再去免疫

类别	实验用途	备注
QuickAntibody-5w	诊断原料、抗体药物等高质量单抗制备	5w抗体效价会更高，且亲和力会更强
QuickAntibody-3w	1、科研用途可以采用3周佐剂； 2、小鼠多抗制备； 3、小鼠多抗做病毒抗体中和实验	3w抗体效价可高达十几万
QuickAntibody-2w	1、小鼠多抗制备； 2、小鼠多抗做病毒抗体中和实验 3、赶时间

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1、用量类问题

1.1说明书里水佐剂全病毒、全细菌免疫抗原用量1-10ug指什么

1.2 佐剂适用物种

1.4 佐剂1ml可以免疫几只小鼠

2、实验步骤相关问题

2.1 如何免疫及冲击免疫

2.2必须使用肌肉免疫么

2.3佐剂使用后鼓包或有流出

2.4能否缩短免疫间隔时间

2.5抗原溶剂

3、产品应用相关问题

3.1能否用于真核表达的质粒或者全病毒

3.2能否用于细胞或病毒等非蛋白类抗原上

3.3能否用于核酸质粒或RNA的免疫

3.4能否用于疫苗研发

4、佐剂沉淀

5、佐剂的成分

6、免疫2针后效价不理想

7、虽然抗体效价达到要求，但是小鼠脾脏没有发生肿大的原因

8、佐剂的区别，如何选择，能否混用

9、水佐剂为何比弗氏佐剂贵

10、能否-20℃保存

11、兔8w佐剂免疫一只兔子的抗体产量

12、弗氏佐剂免疫一针后，后续可否用快速佐剂

13、能否与铝佐剂平行使用