Cleaved-Caspase-9 (D353) Polyclonal Antibody-博奥龙Biodragon

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Catalog_no	BD-PC0012
Product_name	Cleaved-Caspase-9 (D353) Polyclonal Antibody
Category	抗原抗体
Applications	IF,WB,IHC-p,ELISA
Reactivity	Mouse,Rat
Size	100μg/50μg/20μg
Price	2000.00/1100.00/500.00
Gene_name	CASP9
Protein_name	Caspase-9
Rat gene id	58918
Rat swiss prot no	Q9JHK1
immunogen	The antiserum was produced against synthesized peptide derived from human Caspase 9. AA range:323-372
Specificity	Cleaved-Caspase-9 (D353) Polyclonal Antibody detects endogenous levels of fragment of activated Caspase-9 protein resulting from cleavage adjacent to D353.
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source	Rabbit
Dilution	IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration	1 mg/ml
Storage_stability	-20°C/1 year
Msds	MSDS_Antibody.pdf
othername	CASP9; MCH6; Caspase-9; CASP-9; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; APAF-3; ICE-like apoptotic protease 6; ICE-LAP6
Observed_band	17KD
Instructions	查看与下载
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	Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Caspase 9 （Cleaved-Asp353） Antibody. The picture on the right is blocked with the synthesized peptide.
	Western blot analysis of lysates from NIH/3T3 cells, treated with Etoposide 25uM 60‘, using Caspase 9 （Cleaved-Asp353） Antibody. The lane on the right is blocked with the synthesized peptide.
	Immunofluorescence analysis of human-stomach-cancer tissue. 1,Cleaved-Caspase-9 （D353） Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
	Immunofluorescence analysis of human-stomach-cancer tissue. 1,Cleaved-Caspase-9 （D353） Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
	Immunohistochemical analysis of paraffin-embedded Human-testis tissue. 1,Cleaved-Caspase-9 （D353） Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1,Cleaved-Caspase-9 （D353） Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1,Cleaved-Caspase-9 （D353） Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. 1,Cleaved-Caspase-9 （D353） Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.
	Western Blot analysis of various cells using Cleaved-Caspase-9 （D353） Polyclonal Antibody diluted at 1：1000
	Western Blot analysis of HEPG2-UV cells using Cleaved-Caspase-9 （D353） Polyclonal Antibody diluted at 1：1000

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