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| Catalog_no | BD-PC0014 |
| Product_name | Cleaved-Caspase-9 p35 (D315) Polyclonal Antibody |
| Category | 抗原抗体 |
| Applications | WB,IHC-p,IF,ELISA |
| Reactivity | Human |
| Size | 100μg/50μg/20μg |
| Price | 2000.00/1100.00/500.00 |
| Gene_name | CASP9 |
| Protein_name | Caspase-9 |
| Human swiss prot no | 842 |
| Human swiss prot no | P55211 |
| immunogen | The antiserum was produced against synthesized peptide derived from human Caspase 9. AA range:266-315 |
| Specificity | Cleaved-Caspase-9 p35 (D315) Polyclonal Antibody detects endogenous levels of fragment of activated Caspase-9 p35 protein resulting from cleavage adjacent to D315. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Source | Rabbit |
| Dilution | WB 1:500-2000, IHC-p 1:50-300, IF 1:50-300 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage_stability | -20°C/1 year |
| Msds | MSDS_Antibody.pdf |
| othername | CASP9; MCH6; Caspase-9; CASP-9; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; APAF-3; ICE-like apoptotic protease 6; ICE-LAP6 |
| Observed_band | 37KD |
| Instructions | 
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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Caspase 9 （Cleaved-Asp315） Antibody. The picture on the right is blocked with the synthesized peptide. |
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Western blot analysis of lysates from 293 cells, treated with Etoposide 25uM 60‘, using Caspase 9 （Cleaved-Asp315） Antibody. The lane on the right is blocked with the synthesized peptide. |
polbd-p-ihc-human-liver.jpg) |
Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,Cleaved-Caspase-9 p35 （D315） Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only. |
polbd-p-ihc-human-lung.jpg) |
Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1,Cleaved-Caspase-9 p35 （D315） Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only. |
polbd-p-ihc-human-lung-cancer.jpg) |
Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. 1,Cleaved-Caspase-9 p35 （D315） Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only. |
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Immunofluorescence analysis of Human-breast tissue. 1,Cleaved-Caspase-9 p35 （D315） Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B |
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Immunofluorescence analysis of Human-breast tissue. 1,Cleaved-Caspase-9 p35 （D315） Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B |
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Western Blot analysis of various cells using Cleaved-Caspase-9 p35 （D315） Polyclonal Antibody diluted at 1：1000 |
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Western Blot analysis of 293 cells using Cleaved-Caspase-9 p35 （D315） Polyclonal Antibody diluted at 1：1000 |
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